Western Blot: CHO K1 Whole Cell Lysate [NB800-PC13] - Detection of alpha tubulin in lane 1. CHO/K1 Whole Cell Lysate (10 g) was run on a 4-20% gel, then transferred to 0.45 um nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20C, primary antibody was used at 1:1000 overnight at 4C. Anti-Rabbit IgG (H&L) (GOAT) antibody IRDye800CW secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting and imaged on the LiCor Odyssey imaging system. Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for CHO K1 Whole Cell Lysate